Bangs Laboratories -Model PolyLink -Protein Coupling Kit

Carboxyl (COOH) microparticles can be used for covalent coupling of proteins by activating the carboxyl groups with water-soluble carbodiimide. The carbodiimide reacts with the carboxyl group to create an active ester that is reactive toward primary amines on the protein of interest. Bangs Laboratories, Inc. offers the PolyLink Protein Coupling Kit for COOH Microspheres for the covalent coupling of proteins to carboxylated microspheres. The procedure that follows has been optimized for polymer microspheres 1μm or larger. The contents of the kit are sufficient for 50 coupling reactions using 200-500μg of protein per reaction. The kit has been optimized using purified IgG as the coupling protein.