Bangs Laboratories, Inc. products

BioMag® Amine consists of a suspension of magnetic iron oxide particles coated to provide primary amine groups. The amine groups allow for the covalent attachment of proteins or ligands with retention of biological activity. BioMag® Amine may be purchased separately or as a component in the BioMag Magnetic Immobilization Kit, which is designed to introduce the user to the ease of magnetizing biological molecules. The BioMag® Magnetic Immobilization Kit includes BioMag® Amine, glutaraldehyde (the reagent used to couple proteins to BioMag®), a BioMag® Separator, and a Reaction Flask. Supplied with the kit is 10mL of BioMag® suspension, sufficient to attach up to 100mg of most proteins. A coupling efficiency of greater than 80% is achieved for many proteins. Higher amounts of protein can be attached at lower coupling efficiencies.

Carboxyl (COOH) microparticles can be used for covalent coupling of proteins by activating the carboxyl groups with water-soluble carbodiimide. The carbodiimide reacts with the carboxyl group to create an active ester that is reactive toward primary amines on the protein of interest. Bangs Laboratories, Inc. offers the PolyLink Protein Coupling Kit for COOH Microspheres for the covalent coupling of proteins to carboxylated microspheres. The procedure that follows has been optimized for polymer microspheres 1μm or larger. The contents of the kit are sufficient for 50 coupling reactions using 200-500μg of protein per reaction. The kit has been optimized using purified IgG as the coupling protein.