Rockland - Model 400-301-MR2 - Recombinant scFv Anti-Human CD45 Antibody

This scFv fragment representing the functional antigen binding fragment of immunoglobulins is produced in bacterial expression systems. Using biochemical procedures and/or genetic modification (recombinant DNA technology), these fragments can be coupled to compounds and proteins that allow for the detection of these fragments. Examples of such compounds include fluorophores (e.g. fluorescein) and enzymes (e.g. alkaline phosphatase) generating fluorescence. The resultant compounds now have bifunctional properties in that they can bind to their target via the scFv fragment and can be detected via their fluorescence marker. In addition to labeling the scFv with fluorophore markers, the scFv antibody fragment has been coupled to magnetic microparticles allowing for the capture of the scFv target complex in a magnetic field. An scFv fragment retains the complete antigen-binding capability of the whole monoclonal antibody, thus facilitating a unique molecule with the property of being used as either a diagnostic or therapeutic reagent. In addition, the scFv with exquisite specificity and affinity towards a specific target can also be used as a delivery vehicle for radioimmunoimaging and radioimmunotherapy. In addition, the small antigen-binding scFv offer several advantages over a whole antibody molecule in that the smaller size of the scFv allow it to penetrate more rapidly and evenly to tumors and can penetrate the blood brain-barrier. scFv fragments have more rapid clearance from blood, and therefore be coupled with drugs and radionuclides in order to result in low exposure of the healthy tissue. Also, there appears to be no uptake of the scFv by the kidney allowing them to efficiently localize to the tumors, an important property in cancer therapy.

Application Note

Recombinant scFv fragment Anti-Human CD45 Monoclonal Antibody has been tested in ELISA Capture, Flow Cytometry, Immunofluorescence. This antibody has been diluted for recommended use per test. Typically, we use 1×10^6 cells in a 100μL experimental sample (a test). Optimal titers for applications should be determined by the researcher.

Purity/Specificity

The scFv antibody fragment was purified from disrupted Escherichia coli culture by column chromatography.

Storage Condition

Store undiluted at -20° C prior to opening. Protect from prolonged exposure to light.

• Description: Recombinant scFv fragment Anti-Human CD45 (Mouse) Antibody - 400-301-MR2
• Synonyms: PTPRD; CD45R; LCA; L-CA; Leukocyte Common Antigen
• Gene Name: PTPRC
• Tested Applications: ELISA, FC, IF
• Target Species: Human
• Cross Reactivity: Human Leukocytes
• Clonality: Recombinant Monoclonal
• Clone ID: BC8
• Format: IgG1
• Immunogen Type: Native Protein
• Immunogen: Human Peripheral Blood Leukocytes
• Physical State: Liquid (sterile filtered)
• Shipping Condition: Dry Ice
• Expiration: Expiration date is eighteen (18) months from date of opening.
• Concentration: 0.71 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.09% (w/v) Sodium Azide
• Stabilizer: None