Lenti-X - Model SARS-CoV-2 - Universal Pseudovirus Packaging Systems

For your convenience, three plasmids encoding the truncated SARS-CoV-2 spike variants D614G, B.1.1.7, and B.1.351 are included. Additional SARS-CoV-2 spike variant mutants can be created using the provided spike variant sequences as template along with the supplied pEmpty vector in a simple, one-step In-Fusion Snap Assembly reaction.

Lenti-X SARS-CoV-2 Packaging Single Shots (Universal) allow streamlined production of high-titer lentivirus pseudotyped with your SARS-CoV-2 spike protein of interest by providing pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of Lenti-X lentiviral packaging plasmids. Pseudovirus production simply involved diluting a SARS-CoV-2 spike variant expression vector and your transfer vector of choice in water, adding to a tube of single shots, and applying the mixture to 293T cells (e.g., Lenti-X 293T Cells, Cat. # 632180) in a 10-cm dish.

Each kit includes 16 tubes of Lenti-X Packaging Single Shots (Envelope-Free; Cat. # 631297), the pEmpty Vector (Cat. # 631298) for cloning and expression of the desired SARS-CoV-2 spike protein, the Lenti-X SARS-CoV-2 Spike Vector Set (Cat. # 631296), which includes three plasmids encoding for truncated SARS-CoV-2 spike variants D614G, B.1.1.7, and B.1.351 and the Lenti-X Reporter Vector Set (Cat. # 632677). Cat. # 632677 includes two self-inactivating lentiviral transfer vectors encoding ZsGreen1 and firefly luciferase, respectively, under the CMV promoter, to be used as reporters for viral transduction.