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ViGeneron - Model REVeRT - Vector Platform
REVeRT, Reconstitution via mRNA trans-splicing, is our next generation vector platform for transfer of large genes. Messenger RNA (mRNA) splicing is a very efficient cellular process for the seamless ligation of adjacent protein coding sequences, thereby enabling the formation of a functional gene product. The splicing process, which normally takes place on a single pre-mRNA molecule (cis-splicing), can also be used to ligate two separate mRNA molecules. The latter is referred to as mRNA trans-splicing and is the basis of ViGeneron’s REVeRT technology.
REVeRT was developed to overcome the limited genome capacity of AAVs (<5Kb).
This technology offers a breakthrough in gene size limitations using dual AAV vector approaches. REVeRT enables highly efficient and precise reconstitution of two mRNAs encoding the proximal and distal portions of a large protein of interest. The split gene parts are packaged into individual vgAAV vectors, mixed, and co-delivered to the target tissue. REVeRT achieves high reconstitution efficiency at mRNA and protein levels.
- Unique: REVeRT is the dual AAV vector technology working at the mRNA level
- Proprietary: REVeRT technology is fully owned by ViGeneron
- Efficient: REVeRT utilizes the highly efficient cellular splicing machinery
- Specific: REVeRT generates the desired full-length protein with high precision
- Seamless: REVeRT results in seamless ligation of sequences
- Broadly applicable: REVeRT can be used for any disease indication
