Proteome Array Screening Services
Antibody validation, autoantibody target discovery, protein interaction screening and more on HuProt arrays.
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Antibody screening service on HuProt arrays
We offer a fully bespoke service for screening monoclonal and polyclonal antibodies of different species on HuProt arrays, as well as for recombinant antibody formats, single domains or binders based on different scaffolds. Cross-reactivity of antibodies is recognised as a crucial issue. Validation of antibody specificity is now understood to be critical to obtaining correct reproducible data in all techniques where antibodies are used, from Western blotting and cell sorting to immunohistochemistry and immunoprecipitation. Many erroneous literature results have come about through use of poorly validated reagent antibodies. Protein arrays are an important means of validating antibodies for these applications and a very effective way of pinpointing off-target reactivities.
Autoantibody screening service on HuProt arrays
We offer a comprehensive service for analysing autoimmune samples (plasma, serum or cell culture fluid) on HuProt arrays. With a repertoire of 23,000 full length human proteins, HuProt arrays are an ideal platform for autoantigen discovery at the broadest level. In addition to known autoantigens, further targets may be involved in giving rise to hitherto unsuspected autoimmunity.
Further screening services on HuProt arrays
Other proteome-wide screening of protein-protein interactions, RNA/DNA-protein interactions, small-molecule – protein interactions, post-translational modifications or any different interactions can be performed on HuProt arrays, providing an ideal comprehensive platform. Please contact us to discuss the details of your project.
Design of arrays with customised content
If you are interested in a limited selection of defined proteins from the HuProt collection, we can work with you to design customised arrays. Up to 200 proteins can be accommodated, printed in repeated subarrays on the same slide, allowing efficient and economic screening of larger sample numbers. Please contact us to discuss the details of your project.
Our services typically include
- Individual consultation to determine optimal assay design and conditions
- Sample handling, array incubation and processing
- Array scanning and image analysis
- Data analysis using our specialised inhouse developed software
- Full ranked processed data provided for each sample
- Full written report, individual to the requirements of the customer project, including interpretation of the results
Part service options also available
As an alternative to our full service, we can scan and analyse HuProt arrays probed by you.
Please get in touch to discuss the best option.
Applications
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Proteome array screening solutions for protein array sector - Medical / Health Care
The concept behind protein arrays is the immobilisation of sets of proteins, up to very large numbers, on solid supports, to perform miniaturised, multiplexed binding assays with a variety of potential ligands or interactants (see powerpoint presentation on right). HuProt v4 human proteome arrays as used at CPA comprise full length human proteins, many of which will retain their native conformation. HuProt arrays can be distinguished from arrays of peptides, used primarily to identify linear epitopes, or ...
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Proteome array screening solutions for antibody specificity profiling sector - Medical / Health Care
Comprehensive antibody reactivity profiling: validate specificity, identify cross-reactions. The issue of antibody cross-reactivity is one that has often been often overlooked or sidelined in the past, with typical antibody QC involving evidence of binding to the intended target, while neglecting potential cross-reactivity. In recent years, however, many examples have come to light where poor validation of antibodies has led to misleading or erroneous conclusions for assays in which they are used as ...
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Proteome array screening solutions for protein-protein interactions sector - Medical / Health Care
Determine the interactome of your favourite protein. HuProt arrays provide a primary platform for screening proteins for binary interactions with the world`s largest collection of human proteins immobilised on a single microarray. Over 20,000 potential interactions are assessed in a single experiment. Array screening facilitates detection of interactors with low abundance in cellular systems. Combined with other data, the array output can confirm known interactors and discover new ones, illuminating the ...
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Proteome array screening solutions for protein - nucleic acid interactions sector - Medical / Health Care
Identify interacting proteins for your DNA/RNA molecule of interest. Profiling of nucleic acid binding on HuProt human proteome microarrays can reveal interactions with the largest collection of individual human proteins available on a single slide. Nucleic acid-protein interactions regulate many aspects of gene expression and thus cell physiology and pathology. DNA-protein interactions occur via a range of binding motifs present in transcription factors and nuclear receptors (e.g. DNA-binding domains, zinc ...
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