Targeted Bisulfite Sequencing (Methyl-Seq) Service

Targeted Bisulfite Sequencing is an NGS method used to evaluate site-specific DNA methylation changes. The process is similar to pyrosequencing but offers a much higher throughput overall. Our next-generation sequencing platforms can deliver a great amount of useful DNA methylation information with publication-ready data parsed by our expert bioinformatics scientists. The methylation analysis at single-base resolution of individual cytosine in DNA is facilitated by bisulfite treatment of DNA followed by PCR amplification of targeted region, library construction, and sequencing of the amplicon regions. Specific primers are designed for the region of interest and cytosine methylation changes are evaluated within that region. Each DNA methylation site of interest receives a high-sequencing depth of coverage for accurate, quantitative and single-base resolution data output.