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Electroporation Articles & Analysis
10 articles found
Platelets Directly Carry Drugs Platelets can take up drugs into their cells via endocytosis. The traditional electroporation drug loading method suffers from complex procedures and delivers low packaging rates together with significant trauma. ...
That is, blood is first taken, RNA from lymphocytes in the blood is extracted, reverse transcribed into cDNA, and then PCR amplification is performed. After electroporation into competent cells, a phage library is established, and multiple positive clones are obtained through phage display technology.Step 3: Expression and purification of positive clones. ...
Currently, the commonly used methods for introducing biological macromolecules into cells include liposome mediated method, virus vector mediated method, electroporation method and microinjection method, etc. However, these methods have disadvantages such as low transport efficiency, high cytotoxicity, poor safety and poor targeting specificity, which greatly limit their ...
Here we describe a method to enforce expression of such receptors in human NK cells relying on electroporation of mRNA and compare it to retroviral transduction of cDNA. ...
However, the traditional method of loading exogenous substances into exosomes such as electroporation method is too inefficient and easily destroys the integrity and functionality of exosomes, making exosomes face challenges in biomedical applications. ...
However, the disadvantage of physical methods such as electroporation, microinjection, hydrodynamic injection, and microfluidic cell delivery is that it may cause cell death, and the scope of application is relatively ...
The other two are lipid nanoparticles (LNPs), now famous for their roles in mRNA vaccines, and plasmid electroporation. Relative to these other options, viral vectors have the advantage of higher delivery efficiency, better targeting, and a long clinical history starting with their use in vaccines and more recently, in gene therapy. ...
Hydropore™ has a simple installation process with a nearly identical workflow to that of electroporation. For experiments that require multiple electroporation cuvettes, Hydropore™ leads to a lower cost per experiment relative to popular RUO electroporation systems. ...
Gene-modified cell therapies (GMCTs) represent the most effective therapeutic platform for many patients with advanced diseases including relapsed and refractory leukemia, non-Hodgkin lymphoma, and other blood cancers [1]. Specifically, chimeric antigen receptor T cell (CAR-T) therapies targeting CD19 have demonstrated remarkable responses and possibly cures in patients with advanced acute ...
Chimeric antigen receptor T-cells (CAR-T) are one of the first clinically approved therapies to take full advantage of the most significant advancements in immunology and genetic engineering. The resulting therapeutics have been shown to produce remission rates exceeding 80% in patients with CD19+ B-cell lymphoma, making them nothing short of remarkable [1]. Unfortunately, CAR-T cells seem ...