Well Plate Articles & Analysis: Older
11 articles found
MSCs exhibit specific morphology, which makes them unique compared to other cell types typically used in CLD. While forming spindle-shaped, elongated, and transparent cell projections, the MSCs can pose a significant challenge when it comes to imaging and subsequent image analysis. Many imaging systems on the market are simply not fit for precise detection of these unusual cell types. ...
IC50 values were determined using the “log(inhibitor) vs. response -Variable slope (four parameters)” equation in GraphPad Prism version 9.4.0. 2.8 CC50 determination Per well, 2.5 × 104 Caco-2 cells or 4 × 104 Calu-3 cells were seeded in 96-well plates (Greiner Bio-One, Kremsmünster, Austria) and incubated for 24 h ...
The Palpator is a high-throughput mechanical tissue analyzer that measures the mechanical properties of engineered tissues growing in 96- or 384-well plates and allows chemical library screening. IVS has carried out proof-of-concept studies to analyze the molecular mechanisms of action of different compounds on the contractile activities of different muscle ...
Automation now eases manual processes in so many aspects of society, from smart home switches to manufacturing lines. It’s also helping those working in molecular biology, biotech and pharmaceuticals to find new efficiencies and cost savings. So why consider an upgrade from manual pipetting in your lab? ...
TNF-a activation of intestinal epithelia can disrupt the intestinal barrier via mechanisms including that activation of the inflammatory master regulators, including NFkB-induced IL-8 production and secretion, as well as having direct effects on tight junction integrity and cellular apoptosis. A Novel, High-Throughput Platform to Characterize Novel IBD Therapeutics ...
Gradients in extracellular matrix components, as well as matrix stiffness and the presence of physical binding sites, allow the stem cells to establish themselves within these niches in the intestine and coexist with differentiated cells. ...
In the meantime, Altis has developed solutions to increase throughput on the current version of RepliGut®: a two-dimensional tissue construct that can be placed into higher-throughput plate-well formats for screening of different guide RNAs or donor plasmids. ...
Each insert is designed to fit inside the well of a culture plate, making it more effective for growing 3D tissue layers than seeding cells directly on the surface of a plate. ...
Proof of concept high-throughput toxicity screening was achieved with bioprinted kidney organoids in 96-well Corning® Transwell® plates treated for 72 hours with a range of doxorubicin concentrations (0.2 to 25 µM). Analysis of 6-well and 96-well cell viability data suggested that organoids printed in both ...
Assays were conducted in transparent 12-well polystyrene microtitre plates containing the appropriate viral test suspension. Plates were exposed to simulated sunlight at an optical irradiance of 550 Wm−2 (watts per square metre) delivered from a SUNTEST™ CPS+ solar simulator for 6 hours. ...
Mono-, bi-, and tri-layer nanofilm architectures were deposited on 24-, 96-well plate polystyrene surfaces. Surface roughness of nanofilms was determined using atomic force microscopy. ...