physiological condition Articles
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Physiological role for phosphatidic acid in the translocation of the novel protein kinase c apl ii in aplysia neurons
In Aplysia californica, the serotonin-mediated translocation of protein kinase C (PKC) Apl II to neuronal membranes is important for synaptic plasticity. The orthologue of PKC Apl II, PKC, has been reported to require phosphatidic acid (PA) in conjunction with diacylglycerol (DAG) for translocation. We find that PKC Apl II can be synergistically translocated to membranes by the combination of DAG ...
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Simulation models of organisational systems
Organisational systems are complex systems, which are very dependent on the continuous supportive efforts of trained people. At the same time, the complexity of these systems transcends the capacity of individual engineers or groups of engineers. The construction and operation of medium to large organisational systems therefore requires the use of advanced computerised aids. The intensity and ...
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The Pivotal Glutathione Reductase
Glutathione is a tripeptide containing sulfhydryl groups combined with glutamic acid, cysteine and glycine. Glutathione has two forms, reduced (G-SH) and oxidized (G-S-S-G), and reduced glutathione accounts for the vast majority under physiological conditions. Glutathione reductase catalyzes the interconversion between the two types. The coenzyme of this enzyme is NADPH provided by the ...
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How Much Do You Know about Fluorescence Resonance Energy Transfer?
Fluorescent substance and fluorescent labeling technology The compounds upon which fluorescent labeling depends are called fluorescent substances. Fluorescent substances refer to compounds with a conjugated double bond system chemical structure, which can be excited into an excited state when irradiated by ultraviolet light or blue-violet light, and emit fluorescence when returning to the ground ...
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Hydration status and physiological workload of UAE construction workers: A prospective longitudinal observational study
Abstract Background: The objective of the study was to investigate the physiological responses of construction workers labouring in thermally stressful environments in the UAE using Thermal Work Limit (TWL) as a method of environmental risk assessment. Methods: The study was undertaken in May 2006. Aural temperature, fluid intake, and urine specific gravity were recorded and continuous heart ...
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Advanced Proximity Labeling Techniques
Proximity labeling techniques represent a cutting-edge approach wherein a decoy protein of interest is fused with an enzyme capable of indiscriminately labeling neighboring proteins. These labeled proteins are referred to as "prey" proteins. The field of protein labeling has witnessed significant advancements, prominently featuring proximity-tagging enzymes. Among these, Biotin-dependent biotin ...
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The Power of Combining Telemetry and Plethysmography
The study of respiratory function and control of breathing is vital for understanding various physiological and pathological conditions. In preclinical research, gaining accurate and reliable data is essential to make meaningful discoveries and develop potential therapies. Combining implantable telemetry with whole body plethysmography offers a powerful approach that provides researchers with an ...
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Metabolomics in Gut Microbiota Research
With rapid development in sequencing technology, researchers can better assess the effect of the gut microbiome on human health. Human beings have a symbiotic relationship with the microbiota since birth. The environment, proximity to other humans and animals, diet, genetics, and time changes can all affect the microbial composition of our skin, mouth, and intestines. Compared with other ...
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Protein-Protein Interaction Identification Method—Co-immunoprecipitation (Co-IP) and Pull-Down
Proteins control all biological systems in the cell. Although many proteins function independently, most proteins need to interact with other proteins to maintain complete biological activity. Therefore, in order to better understand the function and biological characteristics of intracellular proteins, researchers often need to identify and analyze protein-protein interactions. ...
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Mesothelin: A New Target for Cancer Therapy
Structure and function of mesothelin The MSLN gene is located in chromosome 16p13. It contains 17 exons, with a cDNA length of about 2138bp, has a 1884-bp open reading frame, and encodes the precursor protein of 628 amino acids (69 kDa). This precursor protein can be hydrolyzed by furin protease into two parts: mesothelin in the size of 40 kDa fragments and 31 kDa secreted fragments called ...
By BOC Sciences
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Cutaneous Patches to Monitor Myoelectric Activity of the Gastrointestinal Tract in Postoperative Pediatric Patients
Abstract Purpose: Limited means exist to assess gastrointestinal activity in pediatric patients postoperatively. Recently, myoelectric gastrointestinal activity recorded by cutaneous patches has been shown in adult patients to be predictive of clinical return of gastrointestinal function postoperatively. The aim of this case series is to demonstrate the feasibility of this system in ...
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Protein N-Glycosylation Analysis Strategy and Procedures
The glycan chain is located in the outermost layer of the cell and is the first molecule encountered and recognized by other cells, antibodies, invading viruses and bacteria. N-glycans regulate a wide range of cellular functions, including cell-cell and cell-matrix adhesion, cell proliferation, cell survival and immune system response, by affecting protein folding, recognition, clearance, ...
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Gamma delta T cells in tumor immunotherapy
Immunotherapy, in addition to surgery, chemotherapy, and radiotherapy, has now become the fourth pillar of anticancer treatment, with T cell-based immunotherapy being a successful cancer treatment technique. T cells can be separated into two subpopulations based on the expression of T-cell receptors (TCRs). αβ T cells, expressing expressing αβ TCR, recognize peptide antigens, such as those ...
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Cancer Target: Mesothelin
Mesothelin is a glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein. Its biological function has not yet been clarified, but due to its limited distribution in normal tissues and high expression in some tumor tissues, it is expected to be used in tumor-specific treatment. In existing studies of human epidermoid carcinoma cell lines expressing mesothelin, biodistribution analysis ...
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Cytokine Involvement in Inflammatory Immune Disorders
Cytokines, small molecules with potent biological activity, are primarily synthesized by immune and non-immune cells (e.g., vascular endothelial cells, epidermal cells, fibroblasts) in response to stimulation. These signaling molecules form intricate networks that contribute to immune responses, inflammatory reactions, cell proliferation, and growth. Cytokine Dysregulation in Systemic Immune ...
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Antibody Drug Conjugates: "Biological Missiles" For Targeting Cancer Therapy
Antibody-drug conjugates (ADCs) usually consist of monoclonal antibodies (mAbs) covalently linked to cytotoxic drugs via chemical linkers. With high specific targeting ability and strong killing effect, it has achieved precise and efficient removal of cancer cells, and has become one of the hot spots in the development of anticancer drugs. In 2000, the FDA first approved the ADC drug Mylotarg ...
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ADC Cleavable Linker : Classification and Mechanism of Action
Antibody-drug conjugates (ADCs), as emerging anticancer drugs, can deliver highly cytotoxic molecules directly to cancer cells to kill them. ADC is a monoclonal antibody covalently bound to cytotoxic chemical substances (payload) through linker. ADC linker plays a key role in the therapeutic effect of ADC, and its characteristics greatly affect the therapeutic index, pharmacodynamics and ...
By BOC Sciences
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Evaluating Microbiome Therapies with Intestinal Cell Models
The microbiome has been shown to play roles in many different disease processes and is implicated in many more, from infectious and gastrointestinal diseases to autoimmune and neurological disorders. Advanced models of the gut leveraging intestinal and colonic stem cells are enabling the development of more reliable and robust systems for evaluating the impact of changes in the microbiome on gut ...
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Effect of endothelium mimicking self-assembled nanomatrices on cell adhesion and spreading of human endothelial cells and smooth muscle cells
Abstract The goal of this study is to develop unique native endothelium mimicking nanomatrices and evaluate their effects on adhesion and spreading of human umbilical vein endothelial cells (HUVECs) and aortic smooth muscle cells (AoSMCs). These nanomatrices were developed by self-assembly of peptide amphiphiles (PAs) through a solvent evaporation technique. Three PAs, one containing the ...
By Endomimetics
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Spine-Intra-Abdominal Pressure (SIAP) - Case Study
The SIAP study investigates the effect of using Ergotrics' Inflatable Prone Support (IPS) on intra-abdominal pressure (IAP) during spine surgery. Study background The objective of this study is to demonstrate the ability of the inflatable Prone Supports (IPS) to lower the IAP of patients in the prone position. Whenever the patients’ position is changed to prone, the IAP is increased due to ...
By Ergotrics NV
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